Located on Lake Michigan’s southwest shore, Milwaukee is the largest city in Wisconsin and fifth largest in the Midwest. Milwaukee is known for its breweries and historical attractions and is one of the most ethnically and culturally diverse cities in the nation. A changing climate presents serious threats to the city and risks to the Great Lakes. Situated in a major metropolitan area, flooding and urban heat islands threaten human health, especially in low-income neighborhoods. The urban forest is estimated to have nearly 3.7 million trees and a canopy cover of almost 22%, providing social, economic, and environmental benefits to the local community. Understanding tree species vulnerability and key climate change impacts is critical to Milwaukee’s climate adaptation and management efforts in the 21st century.